Effect of AP-1 mutation on the serum responsiveness and UV inducibility of rat PCNA promoter

• Main Authors: Ho, Jih-Lin, 何季霖
• Other Authors: Liu Yin-Chang
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/66656144018817828369

碩士 === 國立清華大學 === 生命科學系 === 85 === Proliferating cell nuclear antigen (PCNA), anauxiliary factor of DNA polymerase D, is required in DNA replication and excision repair. Previous studies have shown that the rat PCNA promoter possesses the serum responsiveness and UV inducibility.In this thesis, the role of AP-1 site (at -64/-58) in rat PCNA promoter in the aspects of serum responsiveness and UV inducibility was studied. The site-specific AP-1 mutant of rat PCNA promoter was constructed using overlap PCR method. The AP-1 mutant promoter isremarkably less active in comparison with itsrespective wild type promoter. Furthermore, the AP-1 mutant partially loses the serum responsiveness and UVinducibility in the experiments in which the mutant promoter was stably transfected into cells. Resultsof this thesis study suggest that AP-1 site (at -64/-58) is necessary for basal activity and plays significant role serum stimulation and UV induction of the rat PCNA promoter.