Study on the Expression of Retinold-Inducible Gene 1 (RIG1) from Tumor Tissues and Gastric Cancer Cells

• Main Author: 白璁璇
• Other Authors: 姜淑媛
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/14162884134279029620

碩士 === 國防醫學院 === 微生物及免疫學研究所 === 85 === Retinoids are compounds with capability of inducing cellular differentiation. They are effective in the treatment of acute promyelocytic leukemia and head and neck squamous cell cancer when used alone or incombination with other agents. In vitro studies have shown that retinoids suppress the growth of several types of cancer cells through inducing cell cycle arrest, differentiation or apoptosis. Studies from our laboratory have demonstrated the growth suppressive activity of all-trans retinoid acid on SC-M1 human gastric cancer cells. Also, a novel Retinoid-Inducible Gene 1 (RIG1) was isolated from SC-M1 cells treated with all-trans retinoid acid using the technique of mRNA differential display. Nucleotide sequences of the RIG1 cDNA showed 54% homology to a tumor suppressor gene H-REV107-1. To further investigate the biological function of RIG1 gene, I hace first used the technique of semi-quantitatve polymerase chain reaction to analyze and compare expression of the RIG1 gene in 13 pairs of tumor and adjacent normal tissues. The results showed that all investigated tissues expressed the RIG1 gene. Also, nine tumor tissues (six from breast, two from liver and one from stomach) had an increase in the RIG1 mRNA levels compared with that of the adjacent tissues. 　Furthermore, effects of over-expressing the RIG1-EGFP fusion protein were analyzed in TSGH9201 gastric cancer cells. Cells with apoptotic-like morphology were increased in TSGH9201 cells transient expressed the RIG1-EGFP fusion protein for two days. Alternation in gene expression patterns was further analyzed using the technique of Microarray. Expression of RIG1-EGFP fusion protein for two days resulted in increasing expression of seven genes (Heme oxygenase 1, GADD153, hPMS2, APG5, MAD, CDC2 and UVRAG) and decreasing expression of the Glutathione peroxidase 2. These differentially regulated genes are involved in the process of antioxidation, cellular apoptosis and DNA repair. Therefore, overexpression of the RIG1 gene may enhance cellular apoptosis through inducing oxidative stress of TSGH9201 gastric cancer cells.