Analysis of Tetracyclines and β-lactam- Antibiotics by Capillary Eletrophoresis

• Main Authors: Chen, Hsin-Yang, 陳信揚
• Other Authors: Hsieh, You-Zung
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/99122427951964819294

碩士 === 國立交通大學 === 應用化學研究所 === 85 === 　　Capillary electrophoresis is one of important separation technique based on different migration velocities of analytes when a potential is applied. This method has many advantages including high separation efficiency, short analysis time, low consumption of sample, and easy automation. In recent years, the widespread use of antibiotics has resulted led to the emergence of antibiotic-resistant organisms. For this reason, some countries have emphasized on the misusing of antibiotics. Capillary electrophoresis was applied to separate two kinds of antibiotics in this paper. One is tetracyclines, another is β-lactam antibiotics. 　　Seven TCs were completely separated from each other. Also, the effect of adding EDTA and 1,3-Diaminopropane was discussed. The optimum separation condition then was applied to analyze milk. 　　The U.S. Food and Drug Administration (PDA) set "safe levels" for β-lactam antibiotics, including amoxicillin, ampicillin, ceftiofur, cephapirin, cloxacillin, and penicillin. In commercially, penicillin has two forms: penicillin G and penicillin V. In this study, micellar electrokinetic chromatography (MEKC) was developed for determining above seven β-lactam antibiotics. The effect of concentration of surfactant and buffer pH value on the retention behavior and selectivity would be discussed. The optimum separation condition then was applied to analyze animal medicine.