| Summary: | 碩士 === 國立交通大學 === 生物科技研究所 === 85 === Imidase is an industrially important enzyme leading to
the productionof D-4-hydroxyphenylglycine(DHPG). DHPG in turn
is the key intermediate for the manufacture of important
antibiotics such as cefoperazone, cefpiramide, amoxicillin,
aspoxicillin and cefadroxil. To be able to produce DHPG, one
needs an enzyme that can selectively hydrolyze D-4-
hydroxyphenyl hydantoin from its racemic mixure. This enzyme is
imidase, also known as D-hydantoinase, dihydropyrimidinase,
dihydropyrimidine hydrase, and dihydropyrimidine amidohydrolase.
In the past, imidase have been purified from Agrobacterium sp.,
Pseudomonas striata, beef liversand rat livers. While Taiwan is
an major pig exporting country, we trust successful industrial
applications of the imidase from pig livers will provide a
tremendous value to our agriculture sector.
In this thesis, a methodology of purifying imidase from pig
livers hasbeen developed. It has been shown that, indeed, the
pig liver imidase can selectivelycatalyze p-D,L-hydrophenyl
hydantoin which in turn leads to the production of D-
hydrophenylglycine(DHPG). The optimum reaction condition for
the pig liverimidase appears to be at pH 8.5 and 60 C. The
obervation is well in line withthe results shown in the
literature. This research has provided important technical
insights towards successful bioreactor design and scale-up for
theproduction of a wide spectrum of antibiotics.
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