| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 85 === Local and introduced shallot cultivars 'hia-Yi'''hwei-Chia,''
Fu-Chen'','S-28'','Philippine'' and 'Thailand'' were used to study
the infuluence of shoot apex size,number of weeks in heat
therapy and growth regulator for obtaining the highest
propagation rate and best culture condition of producing virus-
free seedings. When shoot apex with one leaf primordium
(approximately 300 m)hadbetter virus-free and survival rate. If
shoot apex culture with heat therapycould increase the virus-
free rate. One handred percent virus-free plantlet could be
obtained when shallot plant subjected to 37 for 16 hours and
32 for 8 hours daily continued for 8 weeks. The best culture
medium for 'Chia-Yi'' shallot was the MS medium with Picloram
0.140 mg/l,and BA 8.6 mg/l, or with Picloram 0.39 mg/l, and TDZ
0.03 mg/l, with NAA1.1 mg/l and BA 5.3 mg/l, as well as with NAA
0.4 mg/l and TDZ 0.3 mg/l. The best culture medium for
'Thailand'' shallot shoot apex was the MS medium with Picloram
0.03 mg/l and TDZ 1.0mg/l. Yield trial in the field and storage
test from virus-free plantletoriginated from shoot apex culture
showed plant hight was better than ordinary plants. Number of
tiller. average plant of number of bulbs per plant, and
singlebulb weight of virus-free plant were higher than ordinary
plant. The bulb weight decereased in ordinary bulbs was three
times higher than the virus-freebulbs after 6 weeks storage was
3 times heavier of that of normal bulb.
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