Studies of Redifferentation from Chrysanthemum(Dendranthema grandiflora(Ramat.)Kitamura)Leaf and Receptacle Callus.

• Main Authors: Chiang, Chun-Ya, 江純雅
• Other Authors: Huang Min-Chang
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/30574782162563375032

碩士 === 國立中興大學 === 園藝學系 === 85 === Callus were induced from leaves or receptacles of Dendranthema grandiflora (Ramat.)Kitamura cultivars, such as ''Shiu Fang Chih Li'', ''Costume'', ''Monami'' and ''Red Small Mum''. The factors affected callus proliferation and shoots regeneration were studies. In addition, shoots regeneration from leaf callus which radiated by gamma ray were compared with unradiated plants. Callus formation from leaves explants were cultured on the medium containing basal MS medium,30 g/l of sugar. 5 mg/l of IAA, 10 mg/l BA, 1 mg/l of 2,4-D, and solified by 8 g/l of Difco Bacto agar. Callus proliferation and shoot regeneration of ''Shiu Fang Chih Li'' and ''Red Small Mum'' were profited when medium containing basal MS medium, 30 g/l of sugar, 10 mg/l of IAA, and 10 mg/l of BA. ''Costume'' callus cultured on basal MS medium, 0.5 or 1 mg/l of IAA,1 mg/l of IAA, 1 mg/l of BA developed more fibrous callus, and had more shoots. Callus growth of ''Monami'' were limited and browning by increasing IAA concen- tration. Medium containing 0.05 or 0.1 mg/l of NAA and 0.5 mg/l of BA was suitaable for ''Monami''callus proliferation and shoots formation. 6 or 8 g/l agar promoted callus growth, medium without plant growth regulators accelerated shoots elonglation and root initated. 30 or 20 g/l sucrose was favorable for plantlets growth in vitro and survival ex vitro. The plant characteristics was different among 4 cultivars. The inflorescence morphology of regenerated plant of ''Costume'' and ''Monami'' were significantly different from stock plants, the regenerated plants of ''Red Small Mum'' callus were more variated on flower number, and ''Shiu Fang Chih Li'' had more variation on petal numbers. The flower numbers, flower diameter, flowering-node numbers, ratio of petal length and width, and ratio of leaf length and width of 4 cultivars had different variation, they resulted the various apperance compared to stock plants. The flowers colour of 4 cultivars were no significantly variations. Callus was stopped grow after radiated by gamma ray. After continued subcultures, ''Shiu Fang Chih Li'' callus which treated by 40 and 80 Gy could not recover growth vigor, and browning to die. ''Shiu Fang Chih Li'' callus which treated below 20 Gy was paused to grow for 4 months, than began to proliferated and regenerate shoots. Most of flower diameter of regenerated plants were small than stock plants, and petals shape was irregular, lack of practical value. Other cultivars radiated with gamma ray, the dose was below 20 Gy. All callus became brown and stopped growth. 7 months later, surface of explants was formmed new callus and growth further, but not regenerated buds yet.