Relationship between genetic structure and riparian habitat of rheophyte Acorus gramineus Soland. (Araceae) in Taiwan

• Main Authors: Liao, Lai-Chen, 廖來成
• Other Authors: Ju-Ying Hsiao
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/89811998121638288631

碩士 === 國立中興大學 === 植物學系 === 85 === Acorus gramineus Soland. (Araceae) is a rheophyte and distributed in southeastern Asia. In Taiwan, it is generally found on rocks and cliffsides along riversides at the lower to middle altidudes of around 200 to 2000 meters. Its populations are restricted in riparian habitats and, therefore, the gene flows between rivers are presumed to be very restricted. The reproduction isolation among rivers are expected to be resulted in the higher genetic differentiations among the plants of different rivers.In the present study, leaf samples were collected from populations along 6 rivers on the western Taiwan and the genetic variation was investigated by employing RAPD molecular markers. A total of 255 samples which belonged to 17 populations were studied. Eighty 10-mer primers were screened and 6 of them which showed better amplification results were selected to analyze all of the samples. Data of thirty-four polymorphic fragments were used in statistical analyses. The results of AMOVA analysis indicated that, of the total variation, 47.04% was attributable to differences among rivers, 16.98% was due to differences among populations within rivers, and 35.97% was attributable to differences among individuals within populations. The result of cluster analysis and principal coordinate analysis revealed that populations of each river formed a distinct cluster. Populations of six rivers were clustered into 3 main groups including northern Shinten river, Miawli region, and central-southern region. Among these 3 main groups, northern Shinten river was the most isolated group. The results of the present study indicated that the population genetic structure of the plants of different rivers were highly diversified.