| Summary: | 碩士 === 國立中興大學 === 植物學系 === 85 === Acorus gramineus Soland. (Araceae) is a rheophyte and
distributed in southeastern Asia. In Taiwan, it is generally
found on rocks and cliffsides along riversides at the lower to
middle altidudes of around 200 to 2000 meters. Its populations
are restricted in riparian habitats and, therefore, the gene
flows between rivers are presumed to be very restricted. The
reproduction isolation among rivers are expected to be resulted
in the higher genetic differentiations among the plants of
different rivers.In the present study, leaf samples were
collected from populations along 6 rivers on the western Taiwan
and the genetic variation was investigated by employing RAPD
molecular markers. A total of 255 samples which belonged to 17
populations were studied. Eighty 10-mer primers were screened
and 6 of them which showed better amplification results were
selected to analyze all of the samples. Data of thirty-four
polymorphic fragments were used in statistical analyses. The
results of AMOVA analysis indicated that, of the total
variation, 47.04% was attributable to differences among rivers,
16.98% was due to differences among populations within rivers,
and 35.97% was attributable to differences among individuals
within populations. The result of cluster analysis and principal
coordinate analysis revealed that populations of each river
formed a distinct cluster. Populations of six rivers were
clustered into 3 main groups including northern Shinten river,
Miawli region, and central-southern region. Among these 3 main
groups, northern Shinten river was the most isolated group. The
results of the present study indicated that the population
genetic structure of the plants of different rivers were highly
diversified.
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