Study of the content for TGF-B1 in the experimentally induced inflammatory periodontal tissues in miniature swines

• Main Authors: Ko, Wen Long, 柯文龍
• Other Authors: Tsai Chi-Cheng
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/06350285236180263210

碩士 === 高雄醫學院 === 牙醫學研究所 === 85 === Transforming growth factor B1是多重功能之分子，它具有對許多 target cells及組織獨特極強烈的作用影響；可經由某些細胞間的互相作 用而促進炎性反應；另外，在極微量下即具有極強的單核吞食細胞趨化作 用，亦可因增加PGE2之產生而促進骨吸收。另一方面，TGF-B1可藉著調整 其他生長因子對MP(Metalloproteinases)及TIMP(tissue inhibitor of metalloproteinases)之表現的機轉來擔任細胞間質更換之極重要角色。 已有研究証實在發炎的牙齦組織細胞內外皆有TGF-B1之存在，其分佈也只 限於有炎性反應的區域。本研究是利用16隻迷你豬牙齒做牙齦炎／牙周炎 之誘導，經ligature三、五、八、十三週後，比較各項臨床指數及黑色菌 落比例之變化，並以酵素免疫分析法測定實驗組及對照組牙齦組織中TGF- B1之含量，期能了解這種介質含量變化與疾病狀態之關係。結果顯示實驗 組TGF-B1之濃度均大於對照組且在第三週時，二者有統計學上的差異；第 三週和第十三週間實驗組TGF-B1之濃度有顯著差異且與ligature時間呈負 相關性。實驗組TGF-B1之濃度與臨床指數CI, GI變化量呈負相關性。綜合 以上結果顯示TGF-B1之濃度隨著實驗性牙周炎症之嚴重程度而有動態變化 ，因此可利用牙齦組織中TGF-B1含量變化來探討牙周組織發炎情形。 TGF-B1 is a multifunctional molecule which has unique and potenteffects on many target cells and tissues. TGF-B1 may promoteinflammatory reaction by certain intercellular interaction. Whereas, TGF-B1 shows strong chemotatic activity for mononuclear phagocytes at extremely low concentrations, also stimulates bone resorption by enchancingproduction of PGE2. On the other hand, TGF-B1 plays a very important role in the regulation of extracellular matrix turnover presumably bymodulating the action of other growth factors on MP (Metalloproteinases)and TIMP (tissue inhibitor of metalloproteinases) expression. TGF-B1 wasidentified intra- and extra- cellulary in the inflamed gingival tissue and thedistribution was associated with areas of inflammation. Sixteen mininature swines were used in this model of experimental gingivitis/periodontitis in our study. The ligatures were placed in situ forperiods of 3, 5, 8 and 13 weeks and then we used ELISA to measure thelevels of TGF-B1 in gingival tissue from experimental group and from control group, with comparsion of clinical periodontal parameter and proportion of black-pigmented Bacteroides. The results revealed that theconcentration of TGF-B1 of experimental group was higher and significantlydifferent in statistics on the period of third week than that of the control group. The concentration of TGF-B1 was significantly different betweenthe period of third week and thirteenth week in experimental group,negatively related to the period of ligature. Furthermore, the concentrationof TGF-B1 was negatively related to the changes of calculus index and gingival index. These data indicate that the concentration of TGF-B1 of gingival tissue exhibited dynamic changes associated with the progression ofexperimental periodontal inflammation. the levels of TGF-B1 in gingival tissue are valuable in detecting the inflammatory reaction of periodontaltissue.