| Summary: | 碩士 === 高雄醫學院 === 牙醫學研究所 === 85 === Transforming growth factor B1是多重功能之分子，它具有對許多
target cells及組織獨特極強烈的作用影響；可經由某些細胞間的互相作
用而促進炎性反應；另外，在極微量下即具有極強的單核吞食細胞趨化作
用，亦可因增加PGE2之產生而促進骨吸收。另一方面，TGF-B1可藉著調整
其他生長因子對MP(Metalloproteinases)及TIMP(tissue inhibitor of
metalloproteinases)之表現的機轉來擔任細胞間質更換之極重要角色。
已有研究証實在發炎的牙齦組織細胞內外皆有TGF-B1之存在，其分佈也只
限於有炎性反應的區域。本研究是利用16隻迷你豬牙齒做牙齦炎／牙周炎
之誘導，經ligature三、五、八、十三週後，比較各項臨床指數及黑色菌
落比例之變化，並以酵素免疫分析法測定實驗組及對照組牙齦組織中TGF-
B1之含量，期能了解這種介質含量變化與疾病狀態之關係。結果顯示實驗
組TGF-B1之濃度均大於對照組且在第三週時，二者有統計學上的差異；第
三週和第十三週間實驗組TGF-B1之濃度有顯著差異且與ligature時間呈負
相關性。實驗組TGF-B1之濃度與臨床指數CI, GI變化量呈負相關性。綜合
以上結果顯示TGF-B1之濃度隨著實驗性牙周炎症之嚴重程度而有動態變化
，因此可利用牙齦組織中TGF-B1含量變化來探討牙周組織發炎情形。
TGF-B1 is a multifunctional molecule which has unique and
potenteffects on many target cells and tissues. TGF-B1 may
promoteinflammatory reaction by certain intercellular
interaction. Whereas, TGF-B1 shows strong chemotatic activity
for mononuclear phagocytes at extremely low concentrations, also
stimulates bone resorption by enchancingproduction of PGE2. On
the other hand, TGF-B1 plays a very important role in the
regulation of extracellular matrix turnover presumably
bymodulating the action of other growth factors on MP
(Metalloproteinases)and TIMP (tissue inhibitor of
metalloproteinases) expression. TGF-B1 wasidentified intra- and
extra- cellulary in the inflamed gingival tissue and
thedistribution was associated with areas of inflammation.
Sixteen mininature swines were used in this model of
experimental gingivitis/periodontitis in our study. The
ligatures were placed in situ forperiods of 3, 5, 8 and 13 weeks
and then we used ELISA to measure thelevels of TGF-B1 in
gingival tissue from experimental group and from control group,
with comparsion of clinical periodontal parameter and proportion
of black-pigmented Bacteroides. The results revealed that
theconcentration of TGF-B1 of experimental group was higher and
significantlydifferent in statistics on the period of third week
than that of the control group. The concentration of TGF-B1 was
significantly different betweenthe period of third week and
thirteenth week in experimental group,negatively related to the
period of ligature. Furthermore, the concentrationof TGF-B1 was
negatively related to the changes of calculus index and gingival
index. These data indicate that the concentration of TGF-B1 of
gingival tissue exhibited dynamic changes associated with the
progression ofexperimental periodontal inflammation. the levels
of TGF-B1 in gingival tissue are valuable in detecting the
inflammatory reaction of periodontaltissue.
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