The Study of N-acetylation Phenotype of Caffeine in Aboriginal

• Main Authors: Wang, Meng-Lin, 王夢麟
• Other Authors: Ko Ying-Chin
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/38197695170185770326

碩士 === 高雄醫學院 === 公共衛生學研究所 === 85 === Acetylation activity is polymorphism. The liver N-acetyl- transferase isgoverned by two major alleles at a single autosomal locus, the phenotype canbe classified as ''slow'' and ''fast''. Caffeine used as a metabolic probe foracetylator phenotyping in Taiwan aborigines. 840 students of 6 middle schoolin aboriginal regions were studied. We collected 2 hour spot urine sampleafter a can of coffee was drank. We determined the acetylator phenotype statusby! measuring the peak area ratio of two caffeine metabolites, 5-acetylamino-6-formylamino-3- methyl-uracil (AFMU) and 1-methylxanthine (1X),using a high- performance liquid chromatographic method. The frequencydistribution of acetylator phenotype had been demonstrated to be trimodal. Theslow acetylator was 48.95% in PaiwanI, 48.81% in Atayal, 29.82% in Ami, 29.17%in PaiwanII, 23.08% in Bunun, 21.26% in Fukien-Taiwanese, 14.81% in Hakka isthe lowest. Aboriginal mixed is 37.29%, between the highest and the lowestfrequency of aborigines. Other mixed is 19.67%, closed to Fukien- Taiwanese.The interethnic difference was related to the status of acetylator phenotype.Genders were relative to AFMU/1X ratio, but smoke, drink, sick and drugs werenot. According to trimodal distribution of acetylator phenotype, thedistribution of genotype of rr (homozygous slow acetylator), Rr (heterozygousacetylator), RR(homozygous rapid acetylator) were consistent withHardy-Weinberg theory of population genetics, except Ami.