| Summary: | 碩士 === 高雄醫學院 === 公共衛生學研究所 === 85 === Acetylation activity is polymorphism. The liver N-acetyl-
transferase isgoverned by two major alleles at a single
autosomal locus, the phenotype canbe classified as ''slow'' and
''fast''. Caffeine used as a metabolic probe foracetylator
phenotyping in Taiwan aborigines. 840 students of 6 middle
schoolin aboriginal regions were studied. We collected 2 hour
spot urine sampleafter a can of coffee was drank. We
determined the acetylator phenotype statusby! measuring the
peak area ratio of two caffeine metabolites,
5-acetylamino-6-formylamino-3- methyl-uracil (AFMU) and
1-methylxanthine (1X),using a high- performance liquid
chromatographic method. The frequencydistribution of
acetylator phenotype had been demonstrated to be trimodal.
Theslow acetylator was 48.95% in PaiwanI, 48.81% in Atayal,
29.82% in Ami, 29.17%in PaiwanII, 23.08% in Bunun, 21.26% in
Fukien-Taiwanese, 14.81% in Hakka isthe lowest. Aboriginal
mixed is 37.29%, between the highest and the lowestfrequency
of aborigines. Other mixed is 19.67%, closed to Fukien-
Taiwanese.The interethnic difference was related to the status
of acetylator phenotype.Genders were relative to AFMU/1X ratio,
but smoke, drink, sick and drugs werenot. According to
trimodal distribution of acetylator phenotype,
thedistribution of genotype of rr (homozygous slow acetylator),
Rr (heterozygousacetylator), RR(homozygous rapid
acetylator) were consistent withHardy-Weinberg theory of
population genetics, except Ami.
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