Improvement of the Stability of Lipid Membrane for Liposome Encapsulated Hemoglobin

• Main Author: 吳振宜
• Other Authors: 鍾次文
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/38058472018102346141

碩士 === 中原大學 === 醫學工程學系 === 85 === To improve the stability of LEH membrane, we change hydrogenated PC/unsaturated egg PC ratio to investigate antioxidation capability of the membrane, and coat PE-PEG to the surface of LEH membrane (PE-PEG/LEH) to investigate the aggregation properties by measuring the viscosity values at different shear rates. After liposome incubated in free radical soluctions, the result show that concentraction of MDA, the products of membrane peroxidation, was reduced from 1.943 mmo1/1 to 1.26 mmo1/1 for 30% of hydrogenated PC/toat1 PC case. The viscosity of PE-PEG/LEH is lower then that of LEH at all of tested shear rates. There are less interactions, by measuring the viscosity among the PE-PEG/LEH with fibrinogen and Gelatin suspensions compared to LEH but adverse effect in observed for Dextran (e.q. the value for PE-PEG/LEH/Dextran 70C 3.60cp, and for LEH/Dextran 70C 1.75cp). Base on the results, we can improve antioxidation capability and reduce aggregation process of LEH by above mention methods.