Protection of vitamin E and glutathione on the morphology change in rat hepatocytes under oxidative stress.

• Main Authors: Wang, Zhen-Yu, 王振宇
• Other Authors: Lii Chong-kuei
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/72079395838241864126

碩士 === 中山醫學院 === 營養科學研究所 === 85 === Drug metabolism and normal metabolism in cellular microenvirement can lead to the production of reactive oxygen species. Once these reactive these reactive oxygen species cannot be eliminated, cells will be damaged and leads to membrane morphological changes, such as the bleb formation. Tocopherol, the important antioxidant molecule of cell membrane, can protect membrane lipids and proteins from oxidative damage. Besides, other intracellular antioxidants, such as glutathione (GSH), is also known to have similar functions as tocopherol. Therefore, in this study, we compare the protective effects of tocopherol and GSH on the cell morphology in rat hepatocytes under oxidative stress. In the first section of this study, by treating cells with tert-butyl hydroperoxides, we examined the effects of tocopherol and GSH on lipid peroxidation, membrane protein thiol depletion, cytoskeleton, and cell blebbing. Results indicated that cell morphological change was not directly related to lipid peroxidation, but was corresponded to membrane protein thiol status. Tocopherol suppresses blebbing formation, however, the effect of glutathione is not significant. In the second part, we further examined the role of intracellular thiol status (including non-protein and protein thiols) on cell blebbing. In this study, hepatocytes were pretreated with L-buthionine sulfoximine following by a thiol oxidizing agent, diamide. Result indicated that protein thiols play a more important role on protecting cell from morphological change than GSH. In conclusions: 1). Lipid peroxidation is not an essential of blebbing formation in hepatocytes, otherwise, membrane protein oxidation may play a more important role. 2). Tocopherol effectively inhibits the polymerization of actin, the depletion of membrane protein thiol, and the morphological changes. However, the protection of GSH on these parameters is less significant than tocopherol is. 3). The oxidation of actin thiols is related to the change of cell morphology.