| Summary: | 碩士 === 中國醫藥學院 === 中國藥學研究所 === 85 === In the present study, we investigated the sedative, analgesic,
anticonvulsive, antipyretic and hypothermic effects of the
various preparations of Paeoniae Alba Radix (PAR, PARa, PARb and
PARc) and Shao-Yao-Kan-Tsao-Tang (SKT, SKTa, SKTb and SKTc) by
pentobarbital-induced hypnosis, acetic acid-induced writhing
response, formalin test and LPS-induced fever. We also detected
the content of the major active constituents (paeoniflorin,
paeonol ) of the various Paeoniae alba radix and Shao-Yao-Kan-
Tsao-Tang.The various preparations of Paeoniae Alba Radix and
Shao-Yao-Kan-Tsao-Tang prolonged the sleeping time induced by
pentobarbital. PAR, PARa, PARb and the various preparations of
Shao-Yao-Kan-Tsao-Tang inhibited the writhing response induced
by acetic acid. PARa, PARb, SKT, SKTa and SKTb inhibited the
early phase produced by formalin. PARa, PARb and the various
preparations of SKT inhibited the late phase produced by
formalin. The various preparations of Paeoniae Alba Radix, SKT,
SKTa and SKTb had hypothermic effect in the normal rats. PARa,
PARb, PARc, SKTa and SKTb inhibited the hyperthermia induced by
LPS. PARa, PARb, SKTa and SKTb prolonged the initiation of
clonic convulsion produced by strychnine.In the HPLC analysis,
the paeoniflorin and paeonol content in PARa, PARb, SKTa and
SKTb were higher than PAR, PARc, SKT and SKTc. Furthermore, the
paeoniflorin and paeonol content in the Shao-Yao-Kan-Tsao-Tang
was higher than those in Paeoniae Alba Radix.In conclusion, the
alcohol and acetic acid preparations of Paeoniae Alba Radix and
Shao-Yao-Kan-Tsao-Tang posessed the better sedative, analgesic,
anticonvulsive, hypothermic and antipyretic effects than the
other preparations. They also contained the higher contents of
the major active constituents. Paeoniflorin and paeonol could be
the active constituents in Paeoniae Alba Radix and Shao-Yao-Kan-
Tsao-Tang.
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