| Summary: | 碩士 === 長庚醫學暨工程學院 === 醫學類 === 85 === Colorectal carcinoma has been listed as the major cause
of death among cancers for most western developed nations, and
as the top 3 or 4 in Taiwan area. Following the progress in
medical techniques in recent years, operative mortality of
colorectal carcinoma is dropped. However, low long-term survival
is still the major concern. So, early diagnosis and prevention
to colorectal carcinoma should be taken into consideration.
Methods performed on clinical diagnosis now, including occult
blood test, endoscopical check, immunostaining , and so on, are
time-consuming and require some other tests to confirm the
results. To get a better and more convenient way for diagnosis,
specific tumor markers should be looked as the best candidate.
Tumor markers such as p53, c-myc, k-ras, TGF-a, ornithine
decarboxylase, blood group antigen and CEA, have been studied,
but the optimal one for early diagnosis of colorectal carcinoma
has not yet found. In 1992, Peng Liang and Arthur B.
Pardee developed a method called mRNA differential display in
which differential display in gene fragments from two different
performing PAGE following RT-PCR amplification of RNA.
This thesis was based on this technique. Normal and tumor
tissues from colorectal carcinoma patients were collected after
surgical removal. RNA was extracted and RT-PCR was performed
with arbitrary primers and oligo-dT primers. Following 138
combinations (46 arbitrary primers and 3 oligo-dT primers), 32
gene fragments with differential display and 26 of which were
successfully reamplified by PCR. Two gene segments in normal
tissues were found to express higher intensity than those in
tumor tissues with the magnitude of about 2 to 10 times.
These two irregular expressions may imply the possibility as
colorectal carcinoma specific markers which requires sequencing
and further characterization of these gene fragments before the
application on clinical trial.
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