| Summary: | 碩士 === 東海大學 === 食品科學系 === 84 === The objectives of this research were to study increasing proline
productivityof Corynebacterium glutamicum by using high
concentration solutes contained in media. It was found that high
concentration of glycerol ( 6% , 20% ) andNaCl ( 3% , 10% )
could stimulate the proline productivity of C. glutamicum.The
proline production in the media containing 8% NaCl was 8 time
more thanthose in the regular media. To optimize the medium
composition, responsesurface methodology ( RSM ) with three-
variable ( X1=ammonium chloride ,X2=NaCl , X3=glucose ) and
five-level central composite design was adopted.It was shown
that the optimal concentration of ammonium chloride , NaCl , and
glucose were 1.4 , 6.9 , 1.76% , respectively. On this
condition , prolineproduction of 72-hour fermentation was 0.1274
umole/ml , and the predictiveproduction was 0.1233 umole/ml. The
difference is insignificant according tot-test (p > 0.05 ). The
optimum medium formila was also applied to 2-liter batch
fermentation in a jar fermenor. The production of proline after
72-hourfermentation was 0.10 umole/ml, approximately 78% of the
production byshaking flask fermentation.
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