Recovery of Intracellular Biological Products by Crossflow Membrane Filtration

• Main Authors: Sue,Hou Chain, 蘇侯謙
• Other Authors: Ju,Shen Chun ; Chan,Lin Lin
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/82951464331908621853

碩士 === 文化大學 === 造紙印刷研究所 === 84 === Using crossflow membrane filtration to recover intracellular biological products had already widely used in biotechnology. In this study, we use two crossflow membrane filtration systems to separate Phytase from the homogenates of r-DNA yeasts. One is low shear rate and laminar flow called Linear Channel system, the other is high shear rate and turbulent flow called NGK system. Crossflow filtration operation parameters such as crossflow velocity, fluid flow type, feed concentration, pH value of suspension, types of membrane, backflushing and the addition of additives were studied. The results showed that increasing crossflow velocity, decreasing feed concentration, increasing pH value of suspension, using UF membrane and backflush in NGK system could improve the recovery yield. We found that when increasing crossflow velocity will decreasing Phytase''s passage. Further, by experiment to prove that particle size distribution of cell-debris cake on membrane surface will effect Phytase''s passage. In NGK system filtration rate is proportional to shear rate''s power of 0.55, in Linear Channel system filtration rate is proportional to shear rate''s power of 0.44. Although when using high shear rate will cause low Phytase''s passage, in all, because of higher filtration rate in high shear rate, using high shear rate to recover Phytase is more advantage.