| Summary: | 碩士 === 文化大學 === 造紙印刷研究所 === 84 === Using crossflow membrane filtration to recover
intracellular biological products had already widely used in
biotechnology. In this study, we use two crossflow
membrane filtration systems to separate Phytase from the
homogenates of r-DNA yeasts. One is low shear rate and laminar
flow called Linear Channel system, the other is high shear
rate and turbulent flow called NGK system. Crossflow
filtration operation parameters such as crossflow velocity,
fluid flow type, feed concentration, pH value of suspension,
types of membrane, backflushing and the addition of additives
were studied. The results showed that increasing crossflow
velocity, decreasing feed concentration, increasing pH value of
suspension, using UF membrane and backflush in NGK system
could improve the recovery yield. We found that when
increasing crossflow velocity will decreasing Phytase''s
passage. Further, by experiment to prove that particle
size distribution of cell-debris cake on membrane surface
will effect Phytase''s passage. In NGK system filtration rate
is proportional to shear rate''s power of 0.55, in
Linear Channel system filtration rate is proportional to
shear rate''s power of 0.44. Although when using high shear
rate will cause low Phytase''s passage, in all, because of
higher filtration rate in high shear rate, using high shear
rate to recover Phytase is more advantage.
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