Study on the properties and stability of IgY from eggs laid by hens immunized with streptococcus mutans

• Main Authors: Chen, Yu Tang, 陳玉堂
• Other Authors: Zhang, Hong Min
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/46024248547257252282

碩士 === 國立臺灣大學 === 食品科技研究所 === 84 === Dental caries is perhaps the most prevalent disease affecting humans today. The cause of this disease has been precisely elucidated, and the preventive methods,active immunization and passive immunization, were suggested by many investigators. Owing to the predominant side effects of active immunization induced by causative bacterium, passive immunization with hen-egg-yolk antibody was preferably favored. Besides this,IgY (immunoglobulin in yolk)content was estimated to be about 10mg/ml yolk, higher than antibody contents in mammalian serum and milk. In this experiment, hens immunized through various routes, such as intramuscular, subcutaneous and oral administration with Streptococcus mutans MT8148 serotype c strain,were investigated to understand the difference of immunization reactions and the changes of yolk and serum antibody values after initial immunization.The cross reactions of IgY against S. mutans serotype a, b, d, e, f and g were also studied.Results showed that the specificity of yolk and serum antibody of IM treated hens was nearly 10 times as high as that of SC treated ones. IgY from the hens immunized with S. mutans MT8148 serotype c strain showed significant cross reaction against serotype e and f, while minor reactions against serotype a, d, g and b were observed. Further purification through column chromatography on Sephacryl S-300, peak 1 was identified to be with higher antibody values based on the results of ELISA assay and precipitation lines of Quchterlony method. IgY from hens immunized with S. mutans was found quite stable at pH4-11.The addition of 50%(w/v)sucrose or invert sugar to IgY solution showed apparent protection against heat denaturation at 50- 60℃under acidic condition(pH 3), or at 70-80℃under neutral pHs.