| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 84 === The measurement of glucosamine content in fungal mycelium by
chemical mehtod was usually used for evaluating fungal biomass
on cereal grains.In this study,chitin of fungal mycelium was
first hydrolyzed by hydrochloric acid,and the resulting
glucosamine was then quantified by colorimetric method. The
results showed tgeat hydrolysis yield under acid condition was
0.945,and recovery yield depended on the difference of strains
varied among 0.70-0.90.When growth of fungal mycelium on cereal
grains was quantified by this method,the presence of
interference glucosamine in the cereal grains interfered with
the accuracy of this measurement.From the ratio of glucosamine
to the dry weight in pure mycelium,glucosamine-to-biomass
conversion factor was determined and was used to measure fungal
mycelium on cereal grains. Results showed that the proportion of
glucosamine to the weight of the mycelium varied with incubation
time, incubation temperature, culture medium and environment in
the fungus grown.When conversion factors were used for the
measurement of mixed mycelium, the accuracy of speices-specific
conversion factors was among 0.73-1.25, and the accuracy of
general conversion factor was 0.70-1.30.Moisture migration was
occurred on rice and corn when they were inoculated with 1 ml
1000000 spores/ ml spore suspension and incubated at 30C, 70RH
for various time. After two days of incubation, total
glucosmaine content of rice and corn substrates were increased
than substrates without inoculation, indicating that under the
growth condition, cereal grains might be unsafe due to the
growth of fungi.
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