Genotyping of Respiratory Syncytial Virus G Protein Gene

• Main Authors: Pin-fei Wei, 衛品妃
• Other Authors: Chuan-Liang Kao
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/92077837606527030140

碩士 === 國立臺灣大學 === 醫事技術學系 === 84 === Respiratory syncytial virus (RSV), a single stranded RNA virus, is the most important respiratory pathogen of infancy and early children. Its presence may be witnessed in most communities in many places by the yearly upsurge of pneumonia, bronchilitis, and tracheobronchitis in the very young children. In order to understand the epidemiological distribution of RSV in Taipei, 135 isolates from National Taiwan University Hospital during 1984-96 were investigated using reverse transcription- polymerase chain reaction(RT-PCR) followed by restriction enzyme mapping. Restriction fragment analysis toward the amplified G gene revealed 10 genotypes for subgroup A ,and Ga1 comes to be the predominant genotype(66.3%). Other genotypes of subgroup A were: Ga2 8%, Ga3 5%, Ga4 5%, Ga5 4%, Ga6 3%, Ga7 3%, Ga8 1%, Ga9 1%, Ga10 3%. The subgroup B virus reveal only 3 genotypes, and the predominant genotype was Gb1(86.5%).The distribution of Gb2, Gb3 were 8% and 5%. Subgroup A showed higher infection rate for male and children under 6 months. In the clinical manifestation, sub- group A patients show higher intensive care needs and longer period to recover from RSV infection while the clinical diagnosis seems to have no distinct differences. This study not only improve our understanding toward the RSV G protein genotypes distribution in Taipei, but also provides a feasible way to identify nosocomial RSV infection.