Effects on the growth and disease resistance of Penaeus monodon juveniles by supplementation of squid viscera meal,algal meals and Negarich

• Main Authors: Su, Lee-Choo, 蘇麗珠
• Other Authors: Liao, Wen-Liang
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/86800174091814546313

碩士 === 國立臺灣大學 === 漁業學系 === 84 === The study was conducted to evaluate the effect of squid visce- ra meal,algal meals and Negarich(wood extraction) supplementation on growth and disease resistance of Penaeus monodon juveniles. The experiment was performed in three parts. Firstly,the effect on growth of P.monodon juveniles by supplement of commercial squ- id viscera meal( squid viscera/ soybean meal=1:1),squid viscera meal( dry squid viscera /soybean meal=1:1) and five algal meals (Ulva,Gracilaria, Nannochloropsis,Chlorella and Spirulina)in diets were investigated.After six weeks feeding trial,the shrimps fed with 2.5% Nannochloropsis diet and 5% commercial squid viscera meal diet had better growth performance than the other groups. Secondly,the optimum requirement of dry squid viscera meal and the two algal meals(Gracilaria and Nannochloropsis) in diets for P.monodon juveniles was designed.Meanwhile,the growth effect of Negarich was also studied.After eight weeks feeding trial,wt the squid viscera meal groups,the 5% commercial squid viscera meal diet displayed the most pronounced effect on growth.It''s found that the growth of shrimps decreased as the amount of dry squid viscera meal increased in diets.The,shrimps fed with 2.5% and 5% Nannochloropsis diets had better growth performance.In addition,1.5%Negarich diet revealed better effect on growth. Thirdly,disease resistance of shrimps fed with Gracilaria, Nannochloropsis and Negarich groups were studied by experiment -ally oral infected with white spot syndrome virus,WSSV.Detection of WSSV in shrimps were proceeding by using the polymerase chain reaction and the WSSV DNA specific primers set,146F1 and 146R1.As a results,within the control groups,the shrimps fed with algal meals and Negarich were not infected with WSSV, while the 5% com- mercial squid viscera meal group had 50% infectivity.Within the experiment infected groups,the shrimps fed with the 1.5% Negarich and 10% Gracilaria diets were not infected with WSSV.