| Summary: | 碩士 === 國立臺灣大學 === 植物病蟲害學系 === 84 === The enhancing factors of SpltNPV was studied. In order to
enhance the virulence of SpltNPV, we mixed several chemicals to
the virus suspension to study the efficacy of enhancement. Our
result revealed that SpltNPV was the most effective one against
the Sl larvae. When we added the chemicals into the medium, the
SL7B cell line could grow when the concentration was 0.01%.
Therefore, we measured the TCID50 of SlECV with or without
chemicals. The results showed several chemicals could lower the
TCID50 of SLECV. The TCID50 of inoculant of SlECV was
3.09×10-8, bur, if we add 0.01 % sorbic acid or boric acid
into the medium,the TCID50 could reduce to 1×10-8. However, 1%
chemicals added to artifical diet were harmless to Sl larvae,
the virus suspension containing 1% chemicals could increase the
mortality of larvae. The mortality of larvae increased with
increasing of chemicals' concentration in the virus suspension,
except the fluorescent brightener 28. In addition to the
chemicals, the granulosis virus（GV）could also enhance the
effect of SpltNPV. The Kao Hsiung Plutella xylostella GV (PxGV)
was the most effective to enhance the virulence of NPV. For
estimating the concentration of GV, we established a method for
purification and quantitation. The concentration of PxGV was
estimated by mixing with latex and was observed by TEM. Better
results were obtained when the latex diluted 100X. Absorbance
of the PxGV mixture was measured by a spectrophotometer. The
absorbance peak located between 200-300nm. The OD of different
concentrations of PxGV were estimated under various
wavelengths. The absorbance of PxGV increase with the
concentration increasing and there was a significant
relationship between absorbance and PxGV concentration. When
comparing the absorbance at different wavelengths, the
absorbance decrease with an increase of wavelength to estimate
the concentration of PxGV.
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