Gene Expression Of Carp Egg Membrane Proteins During Oogenesis

• Main Authors: Chen,Jun-Chin, 陳俊欽
• Other Authors: Chang,Yea-Shia;Huang,Fore-Lien
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/66030645034640366728

碩士 === 國立臺灣大學 === 生化科學研究所 === 84 === This essay explored the gene expression of carp egg membrane proteins during oogenesis. According to the sizes, five groups of carp oocytes were classified:perinucleolus stage, oil droplet- yolk vesicle stage, primary yolk stage, secondary yolk stage and tertiary yolk stage. Total RNA was first extracted from carp oocytes in order to quantify the ZP2、ZP3 and fibroin- like protein mRNA by Northern blot analysis. Secondly, the PBS- insoluble fraction was prepared from carp oocytes to quantify the protein content of ZP2、ZP3 and fibroin-like protein by immunodot blot. The mRNA content of ZP2 and ZP3 decreased rapidly when oocytes developed from the perinucleolus stage to the primary yolk stage, but changed slightly during the development from the primary yolk stage to the tertiary yolk stage.The amount of ZP2 and ZP3 mRNA dropped from 1.0 ng and 1.5 ng in one oocyte of the perinucleolus stage to 0.2 ng and 0.2 ng in one oocyte of the primary yolk stage, respectively. The mRNA content of fibroin -like protein also decreased markedly from 1.6 ng in one oocyte of the perinucleolus stage to 0.3 ng in one oocyte of the secondary yolk stage. Thereafter, the fibroin-like protein mRNA content raised when oocytes developed to tertiary yolk stage. On the other hand, the protein content of ZP2 and ZP3 increased slowly when oocytes developed from the perinucleolus stage to the primary yolk stage but increased 180 fold and 279 fold during the later stages of oogenesis, respectively. The fibroin-like protein increased significantly (about 53 fold) until the development from the secondary to the tertiary yolk stage.In summary, the transcription of carp ZP2、ZP3 and fibroin-like protein genes occurs in the early phase of oogenesis and their transcripts are translated into egg membrane proteins until vitellogenesis.