| Summary: | 碩士 === 國立臺灣師範大學 === 生物學系 === 84 === australe (Fr. ) Pat. was investigated by polymerase chain
reaction,restriction fragment length polymorphism, and DNA
sequencing.Two cultures of G. applanatum (Pers. ex Grau) Pat.
CBS 187.31 and G. neo-japonicum Imaz. CCRC 36049 were selected
for comparative studies. By using conserved primers
complementary to rRNA genes of Saccharomyces cerevisiae, a 2.1
Kb fragment containing 17S rRNA gene and a 1.85 Kb fragment
containing 5` half of 25S rRNA gene were efficiently amplified.
Electrophoresis of HhaI, AvaII or HinfI digested PCR products
produced restriction phenotypes. The two intersterility groups
of G. arstrale can be differentiated by AvaII and HinfI
restriction phenotypes. Detail mapping of restriction
sites within rDNA locus by partial digestion and Southern
hybridization reveals conserved and variable restriction sites
for each species or group. The regions containing internal
transcribed spacers were further amplified and subcloned into
pGEM-T for DNA sequencing. Phylogenetic structures were
constructed from analysis of ITS regions by PAUP and MEGA
programs. Our data suggest the two groups of G. australe are
genetically differentiated.
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