Further Study of Schedule-Induced Polydipsia

• Main Authors: Tzi- Fei Shen, 沈子斐
• Other Authors: Che Se Tung
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/03631559402696199737

碩士 === 國防醫學院 === 生理學研究所 === 84 === (1)This study was designed to examine the function of buccal sensory afferents in Schedule-induced polydipsia (SIP) rats. In Experiment 1, SIP rats were smeared over oral cavity with lidocaine（4% w/v）to anesthetize buccal sensory fibers. In Experiment 2, rats were deprived of water to establish dehydration -induced polydipsia（DIP）. Dehydrated rats were smeared with lidocaine, too. In Experiment 3, rats were deprived of oral C fibers with capsaicin pretreatment. Our data showed, SIP rats with the lidocaine treatment had a significant increase in number of licks , water intake.（2）The performance of SIP in combination with the locomotor activity were employed to investigate the physiological effects and interactions of arecoline and amphetamine. Rats received arecoline and amphetamine respectively or in combination were operated to determine the locomotor activity. Our data revealed that amphetamine but not arecoline promoted locomotor activity. However, amphetamine (1mg/kg) enhancement of locomotor activity was attenuated by arecoline (1mg/kg) pretreatment. SIP rats exhibited significant licks and water intakes after receiving arecoline. However, scopolamine（0.1 mg/kg）, reversed this effect. This finding suggests that the influence of arecoline on SIP is acting via muscarinic receptors. On the other hand, amphetamine increased nose pokes at 1 mg/kg doses, while decreased licks and water intakes at 2 mg/kg doses.