Using lipase to study transesterification synthesis of (S)- Naproxen prodrug in organic solvent

• Main Authors: Tsai, Ching Sian, 蔡慶憲
• Other Authors: Tsai Shau Wei
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/15336876422091872027

碩士 === 國立成功大學 === 化學工程學系 === 84 === Past studies on using Candida cylindracea lipase as the catalyst and 4-(2-hydroxyethyl)morpholine as alchol substrate in isooctane at 37 ℃ revealed that the enzyme had good reactivity and selectivity to the enantioselective esterification and synthesis of (S)-Naproxen ester prodrugs. However, due to the low solubility of Naproxen and hence the yield of the required product, in isooctane, enhancement of the substrate solubility and yield of (S)- Naproxen prodrug by using transesterification was proposed. Experimental results indicated that more than 8000 times of the solubility of 2,2,2-trifluoroethyl Naproxen ester compared with that of Naproxen in isooctane was found .The lipase showed good reactivity and enantioselectivity at 37 ℃. Moreover, the kinetics of enzyme was investigated to fit parts of parameter in the model and the steps for product recovery were also addressed.