| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 84 === Hepatic disease, either primary or secondary, occurred fre
quently in the dog. Often few clinical signs indicating hepatic
dysfunction was found during physical examination of the dog.
Thus, liver function tests must be used to evaluate the extent
of disease. The greatest concentration of alkaline phosphatase
（ALP,EC 3.1.3.1.） were found in bone, liver, intestine and
the placenta. Because of this wide distribution, limited
information could be obtained from a total ALP assay. In the
dog, ALP had high sensitivity but low specificity as a test for
liver disease. Electrophoresis could overcome the imperfec
tion. The tissue sources of elevated ALP in serum must be
identified the isoenzyme. The isoenzymes of ALP differed in
their physiochemical and electrophoretic properties, and by
taking advantage of these differences, the individual isoen
zymes could be identified. A number of laboratory procedures
had been used for the experimental identify of the canine ALP
isoenzymes, these include heat inactivation, induce with
cortisone and cases assay. The four identifiable isoenzymes in
canine serum included bone, liver, and glucocortcoid-
induced isoenzymes and an isoenzyme of unknown origin. The
isoenzymes of placental, renal and intestinal ALP had not been
observed in the serum of dogs. We separated isoenzymes of
ALP in sera of normal pup's （ages 1-8 months）. Serum ALP
activity was 150.5(12.8U/L, liver isoenzyme activity was
94.9(11.4U/L（64.3(4.9%）and bone isoenzyme activity
was 55.6(8.9U/L（35.7(4.9%）. Serum ALP activity was
61.9(4.5U/L in normal adult dogs, liver isoenzyme activity
was 56.0(4.0U/L（92.3(1.3%） and bone isoenzyme activity
was 6.0(1.2U/L（7.7(1.3%）. The heat inactivation of canine
serum ALP was shown, without specificity. Canine bone and
the liver alkaline phosphatase were caused more greater
separation without neuraminidase. It was concluded that the
initial increasing of serum ALP activity in dogs were treated
with corticosteroids apparently was a result of increased
synthesis of normal hepatic enzyme. The late-term increased
of serum ALP activity due to the induction of a new isoenzyme.
The sensitivity of electrophoretic temperature was difference
between glucocortcoid-induced isoenzyme and bone
isoenzyme. The data generated could be used as a clinical tool
in the diagnosis of chshing's syndrome.
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