| Summary: | 碩士 === 國立中興大學 === 獸醫微生物學研究所 === 84 === Fluoroquinolone (including Danofloxacin, Ofloxacin and
Enrofloxacin) are widely used in controlling gram negative
bacterial and mycoplasmal infectionsof livestock in Taiwan.
Howere,the problem of drug residues will arise in meat hygiene
if the farmers ignore the withdrawing peroid of these drugs
before slaughtering their livestock. In this study, we
determined the optimum conditionfor the application of high
performance liquid chromatography(HPLC) to the analysis of drug
residue in meat and withdrawing period. In the first part of our
study, muscles and livers were taken from chickens
experimentally treatedwith Danofloxacin, Ofloxacin and
Enrofloxacin in drinking water at dosage of 10,10 and 5 mg/kg
wt./day, respectively. Samples from the chickens of the three
treated groups were pretreated with 0.5M dipotassium hydrogen
phosphate solution, extracted with dichloromethane and then
fractionated in 1% phosphobic acid.The last fraction was assayed
for traces of the drug by HPLC. By comparing the results of the
three tested fluoquinolones, the appropriate withdrawing period
of Danofloxacin, Ofloxacin and Enrofloxacin were found to be 3,
7 and 9 days,respectively. These periods were the same as those
recommended by the drug manufacturers. Since the drug residues
were found to be below 0.001ug per gramof the samples tested, it
is thought that the meat will be safe for human comsumption. In
the second part of this study, of the three hundred muscle
samplescollected from a commercial slaughterhouse that were
assayed for drug residue,fluoroquinolones were observed in 104
muscles(34.67% of tested samples) at concentration ranging from
0.008 to 0.0122 ug/g. The number of the farms with chickens
positive for drug residues were 11 of 15 farms surveyed(73.33%).
Theestablishment of this drug residue assay method will make it
possible for assaying routinely a large number of samples at the
slauterhouse. In addition, our resultson the withdrawing period
and the testing of market samples will provide the Department of
Agriculture r reference for improving meat hygiene and the
strengthening of public health.
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