| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 84 === Summary The clusis analysis with both the
morphological traits and the RAPD marker data were used to
identify 16 bermudagrass cultivars. It was the first time RAPD
method been used on bermudagrass for cultivar identification.
The goals for this experiment are: 1)To build up a database for
bermudagrass morphological characters, and use the data for
cluster analysis to construct relationship data between
cultivars. 2)Testing the reliability of RAPD test compare to the
morphological traits. 3)Develop an accurate and rapid
techniquefor cultivar identification. The morphological
traits were measured based on the categories for
Gramineaegrasses. Both qualitative and quantitative characters
including: ligual, hairs on the edges of collar, pubescence on
leaf blade and on leaf back, stem color,plant height, texture of
the leaf and the canopy of the grass were used to describe the
characteristics of 16 bermudagrass collected. We quantitized
those qualitative characters then added up with the quantitative
charactersfor similarity cluster analysis. With the cluster
analysis, 16 bermudagrass cultivars were separated into three
groups. Selected from 100 primers tested, four were chosen to
be useful forcultivar identification using RAPD marker. Four
primers were able to produce polymorphic DNA fragments to
clearly identify N1, 0035 and 0036 varieties.With cluster
analysis, 16 bermudagrass cultivars were separated into
threegroups. Menbers of the three groups are corresponding to
three types of bermudagrass according to their plant heitht and
genetic constituent. The use of RAPD marker data for
bermudagrass identification were proved to be useful by
comparing the results with morphological traits data. Except for
Tifway,15 cultivars were easily categorized into three types
(common, dwarf, or semi-dwarf) by either RAPD or morphological
traits method. The primerbank may need to be expanded for the
selection of right primer used to separate every bermudagrass
cultivars. From our experiments, use of the RAPD marker for
cultivar identification is a quick and reliable method. A large
scale selection of primers suitable for RAPD on bermudagrass has
to be held before the method can be efficiently used.
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