| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 84 === This research focuses on the use of cruciferous vegetables
as a model system to establish the gene transfer technology,
and to study the possibility for improvement of cruciferous
vegetable with bacteria, insect and frost resistance, through
the art of genetic engineering. The results are summarized as
following: We had constructed the D-amino acid oxidase gene
(DAO) isolated from Trigonopsis variabilis with the promoter of
CaMV 35S, rubisco small subunit (rbcS). The constructed plasmids
included δ-toxin gene of Bacillus thuringiensis (Bt) and
antifreezing protein gene (AF) were transfer into hypocotyl and
cotyledon of cabbage (New top, K-Y cross) and Chinese cabbage
(Tropical pride, New No. 38) via Agrobacterium mediated
transformation. Regenerated plants of two Brassica
vegetables were obtained after transformation with six kinds of
plasmids. The regeneration rate of transformation in Cabbage was
1.4% to 4.8% , in Chinese cabbage was 0.24% to 0.7%. The
transformed plants were examined by PCR, southern and northern
blot hybridization. The results indicated that the expression of
constructed genes was little higher in transgenic plants
transferred with rbcS as promoter than CaMV 35S promoter and did
not obvious. High biological activity in killing Plutella
xylostellac was show in Bt-transformed plants.
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