Construction of a linkage map based on RAPD (Random Amplified Polymorphic DNA)markers in Cucumis melo L.

• Main Authors: Hou, ya-wen, 侯雅文
• Other Authors: Lan ching-lung
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/71442632451340748353

碩士 === 輔仁大學 === 生物學系 === 84 === This research project is intended to construct a RAPD (random amplified polymorphic DNAs) linkage map of Cucumis melo L. Conditions such as the amount of DNA template,the concentration of Mg+2 ions available,the annealing temperature of the primer used and its duration were optimized to generate reproducible RAPDpatterns.Furthermore,a F2 population was established from a F1 plant obtained from a cross of a Eastern melon ♀ parent (cv. SLK-V-052) and a musk melon ♂ parent (cv. PI-414723) .Fourty five prescreened from 300 random 10-mer oligonucleotides primers (with GC% between 50% and 90%) were able to produce DNA polymorphisms among parental lines and F1 in a preliminary survey. Their segregation patterns in RAPDs were subsequently examined on a F2 mapping population comprising of 98 individuals. So far, 20 polymorphic RAPD markers were analyzed. The mapping distances of 19 RAPD markers were computed using the software "Linkage1" and their plausible map locations were determined.In summary, 7 linkage groups spanning 422.61 cM was recognized.