| Summary: | 碩士 === 輔仁大學 === 生物學系 === 84 === This research project is intended to construct a RAPD (random
amplified polymorphic DNAs) linkage map of Cucumis melo L.
Conditions such as the amount of DNA template,the concentration
of Mg+2 ions available,the annealing temperature of the primer
used and its duration were optimized to generate reproducible
RAPDpatterns.Furthermore,a F2 population was established from a
F1 plant obtained from a cross of a Eastern melon ♀ parent (cv.
SLK-V-052) and a musk melon ♂ parent (cv. PI-414723) .Fourty
five prescreened from 300 random 10-mer oligonucleotides primers
(with GC% between 50% and 90%) were able to produce DNA
polymorphisms among parental lines and F1 in a preliminary
survey. Their segregation patterns in RAPDs were subsequently
examined on a F2 mapping population comprising of 98
individuals. So far, 20 polymorphic RAPD markers were analyzed.
The mapping distances of 19 RAPD markers were computed using the
software "Linkage1" and their plausible map locations were
determined.In summary, 7 linkage groups spanning 422.61 cM was
recognized.
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