| Summary: | 碩士 === 中山醫學院 === 生物化學研究所 === 84 === Immunofluorescece analysis using a variety of human autoimmune
sera showed a typical dot-like nuclear staining,called nuclear
domain(ND)10,also desc-ribed as POD or Kr bodies,suggesting that
this structure consists of seve-ral proteins.Two of the ND10
proteins,Sp100 and PML are genetically chara-
cterized and show homology with several transcription factors;
the first a-utoantigen predominant in patients with primary
biliary cirrhosis(PBC) and the second transformation and cell
growth suppressing protein aberrantly expressed in
promyelocytic leukemia cells.In this study,we found a sarcoi-
dosis patient whose serum recognized two proteins with Mr of 92
kDa and 87 kDa in HeLa cell and lymphocyte cell lysate.The serum
also recognized two proteins with Mr of 70 kDa and 66 kDa in rat
liver cell nuclear extract.W- hen 60-100% ammonium sulfate was
added to rat liver cell nuclear extract,a protein with Mr of 58
kDa was recognized by this patient's serum.By the u- sing of
DE-52 cellulose,phenyl-agarose and Superdex HR200 chromatography
a protein with Mr of 58 kDa which againsted the serum of
sarcoidosis patient was purified from rat liver cell nuclear
extract.To determine the NH2-amino acids sequence of this 58 kDa
protein is doing now.
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