| Summary: | 碩士 === 中國醫藥學院 === 中國藥學研究所 === 84 === Whang-Po ( Phellodendron Wilsonii HAY. et KANEHIRA ) was first
recorded in Shen-Nung-Pen-Ts''ao-Ching under the inferior
category and it was changed in Pen-Ts''ao-Gang-Mu under the
middle forest category. Whang-Po has been used as a medicinal
herb in China for clearing away damp-heat from the lower-jiao,
purging fire, eliminating toxic materials, and killing
parasites, and for the treatments of jaundice, metrorrhagia, and
leucorrhagia. It is also important to lower the fire and cure
wilting pattern. The purpose of this study was to establish the
tissue culture techniques of Whang-Po and to seek the
possibility of berberine formation from callus or cell
suspension culture. Experimental results indicated that petiole
was the best explant source for callus induction followed by
leaf blade with midrib and leaf blade without midrib. The
addition of 2 mg/l 2,4-D to the medium containing MS basal salts
was effective in inducing callus formation while the addition of
NAA showed no positive effect. The supplement of BA with the
presence of 2,4-D increased the callus induction rate.
Subculture of the callus on the original callus-induction medium
resulted in accelerated aging and significant senescence.
However, lowering the basal salt concentration in the medium, e.
g., transferring the callus from MS to WPM medium, could promote
the regrowth of the cultured callus. It was also recorded that
callus induced from medium containing 0.5 mg/l 2,4-D showed the
highest rate of regrowth upon transferring to WPM medium.The
most suitable medium composition for subculture of callus was
WPM basal salts supplemented with 2 mg/l 2,4-D, 1 mg/l BA, 1,000
mg/l casein hydrolysate, 10 % coconut milk, 3 % sucrose, and 1 %
agar, with a pH value of 5.2. The interval of subcultures was
between 10 to 20 days.
|
|---|
