| Summary: | 碩士 === 國立臺灣大學 === 職業醫學與工業衛生研究所 === 83 ===
The purpose of this study is to quantitatively determine the urinary metabolites of Chlorpyrifos, and evaluate the precision, accuracy, convenience and economics of the high performance liquid chromatography and biochemistry auto-analyzer which have been used to analyze creatinine, and find out a reasonally performed biological monitoring method.
The Wistar female and male rats were used in this study. The doses administered were 25, 35, 50mg/kg respectively by intraperitoneal (I.P), oral and skin spread to three group, each has eight rats. The urine samples were collected 8, 24, 48 hours after the begining of treatment, then the samples were used for measuring 3, 5, 6-TPC and creatinine by HPLC and measuring creatinine by biochemistry auto-analyzer.
After creatinine calibrated, no matter imtraperitoneal, oral, skin spread or gender, the metabolic concentrations of Chlorpyrifos show 24 hours' data are greater than 8 and 48 hours, 50>35>25mg/kg, but in oral dose female rats＞ male rats.
Compairing the HPLC and biochemistry auto-analyzer methods, the results show no significance between these two methods. HPLC is better than biochemistry auto-analyzer in precision and accuracy, but biochemistry auto analyzer is more convenient and saves more time than HPLC.
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