| Summary: | 碩士 === 國立臺灣大學 === 免疫學研究所 === 83 === Tumor associated antigens (TAAs) were defined as an unique
antigen which were identified in tumor or benign cell but not in
normal one. They could be recognized by T cells via the presen-
tation of MHC molecules. There are many evidence to demonstrate
that the CD8+ cytotoxic T lymphocyte (CTL) play an important ro-
le in the process of tumor rejection. In addition, the expressi-
on of MHC class I molecules on tumor cells is corelated to the
ability of TAA presentation. Based on these concepts, we used RL
♂-1, a T cell leukemia cell line derived from BALB/c strain mo-
use which was accepted by BALB/c mice but rejected by (BALB/c×B
6) F1 mouse, to study the relationship between TAAs and tumor r-
ejection.
It has been documented that pRL1 peptide was one of the TAAs
in RL♂-1 which contained 8 amino acid and was restricted by H-
2Ld molecule. In the present expriments, we demonstrate the spe-
cific T cell response could be induced in BALB/c mice after inj-
ection of pRL1 peptide. It was then extended to establish the pR
L1 specific T cell clones. The obtaintion of a specific CD8+ T
cell lines have been sure through several restimulation in vitro
and FACScan analysis, on the other word, the vaccination potent-
ial of pRL1 peptide was examed. After heavy immunization, the t-
umor was performed on unprimed BALB/c mice but was regressed ev-
en rejected by peptide-primed BALB/c mice.
In the future, the application of these CD8+ T ell lines and
the vaccination effect of the pRL1 peptide should be improved f-
rom the experimental design. And then, it will be expected to a-
pply in the tumor immunology of human cancer therapy.
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