The study of ∝-crystallin from the walking catfish (clarias batrachus):gene and protein characterization

• Main Authors: Zhao, Yan Kai, 趙彥凱
• Other Authors: Qiu, Shi Hong
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/59148904890615924698

碩士 === 國立臺灣大學 === 生化科學研究所 === 83 === αB-crystallin,shown to be a member of the small heat-shock protein( sHSP )family,is synthesized at various developmental stages,especially in response to stress and in a number of pathological states. Clonging and Northern analysis show unambiguously that αB-crystallin mRNA is present in some mature tissues of the walking catfish(Clarias batrachus),although there are some differences in the transcription pattern. Interestingly,sequence analysis has shown that walking catfish αB-crystallin exists as a shortened gene both in cDNA and genomic structure despite its being similar to sHSP as other mammalian αB-crystallins. We have prepared anti-walking catfishαB and αA antiserum,and use them for Western-blot analysis of α-crystallin from various species to study the immunological specificity and relationship. In this study,we have found some differences in the chaperone activity of walking catfish and porcine α-crystallins,which may reflect the structural differences as revealed by the shortened primary sequence in the walking catfish as compared to mammalian αB-crystallins.