Neurogenic vascular extravasation and goblet cell secretion in tracheal mucosa in adult rats neonatally pretreated with capsaicin

• Main Authors: Hwang, Shwu Jing, 黃淑菁
• Other Authors: Huang, Hung Tu
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/51696858300680386170

碩士 === 國立中山大學 === 生命科學研究所 === 83 === Acute inflammation caused by stimulating sensory nerves is called 〝neurogenic inflammation〞. Neurogenic inflammation occurs when the supplying sensory axons are stimulated by irritant substances. The syndromes of neurogenic inflammation in airways include increased vascular permeability, degranulation of goblet cells。 The vagal sensory axon release substance P which can exerts the permeability－increasing effect on the venular endothelium and mucus－discharging effect on the goblet cells. Sensory nerves can be destroyed by treating the neonates with high dose of capsaicin（50 mg/kg）. In this present study we sought to know if neonatal capsaicin could affect the neurogenic plasma extravasation and the mucous secretion of goblet cells in the adults. Capsaicin（90 ug/kg） or substance P（3 ug/kg）was injected to test the permeability of venules and mucus－secreting ability in the airways of adult rats that had neonatally been pretreated with capsaicin. Indian ink was used to label the leaky venules and to measure their area density in the whole mounts. To investigate cell and tissue responses of the mucosa, histological methods were employed. The area density of Indian ink－labeled venules decreased by 69﹪. Extravasation by substance P did not decrease. These findings suggest that neonatal capsaicin could not inhibit the development of microvasculature and the number of venules did not decrease. Histological study indicated that the development of mucosal surface epithelium in trachea of adult rats, after neonatal capsaicin treatment was changed. There was only a few goblet cells. The number of ciliated cell was reduced. These observations suggest that mucus－secreting function was impaired in the airways in rats neonatally treated with capsaicin.