Isolation and Characterization of GP-83 encoding cDNA from cDNA library of human epididymis

• Main Authors: Huan-Yi Tseng, 曾煥怡
• Other Authors: Hwan-Wun Liu ;Yaw-Wen Guo
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/74242961586346401682

碩士 === 國防醫學院 === 解剖學研究所 === 83 === Mammalian sperm are immature and incapable of fertilization when they just emerge from the testis. During the subsequent passage in epididymis, sperm interact with epididymal secretions, and acquire forward motility and ability to recognize and penetrate zona pellucida of eggs. Several genes of sperm maturation-related proteins, such as PH-30, EAP-1, HE 2 and 3 have been isolated and sequenced. DNA sequence of these molecules reveal proteinase- and disintegrin-encoding domains which are the same as those encoded in the genes of a variety of snake venoms. Our previous studies identified a sperm maturation-related glycoproteins, GP-83, in human epididymis. It was secreted from corpus of epididymis will associated in sperm surface. In order to analyze DNA sequence and predict the biological significance, GP-83 encoding cDNA was isolated from cDNA library of human epididymis by immunoscreening using GP-83 specific antiserum. Three positive clones containing inserts of 1.8 Kb, 1.5 kb and 4.0 kb were isola ted. Partial sequences of these inserts, up to 300-400 bp from 3' end, did not reveal any significant homology to known genes in the GenBank. In cDNA library of snake venomous gland, however, an ancrod-like sequence was identified. In conclusion, GP-83 may be a human epididymis-specific protein containing disintegrin-like domain.