Repair of Articular Cartilage Defects with Periosteum

• Main Author: 謝博欽
• Other Authors: 張冠諒
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/04190156834039351305

碩士 === 國立成功大學 === 醫學工程研究所 === 83 === 　　Periosteal grafts have bee used to repair large osteochondral defects in rabbit articular cartilage but early degeneration of the new cartilage has hindered extensive clinical application of this procedure. The effect of GTF-β introduced either by pre-incubation of the periosteum in vitro or by intraarticular injection in vivo was compared to determine the relative effects of these two methods of delivery on the generation of new cartilage. 　　Sixty-four rabbits were divided into five groups: 1. non-grafted group; 2 grafted group; 3. 100ng/ml rhTGFβ1 injected group; 5. grafted and 200ng/100ul rhTGF-β1 injected group. The rabbits were sacrificed at 2 to 12 weeks, the histologic slides were stained with Safranin O and were scored based on a new subjective scoring system. The grafted group healed with hyaline cartilage -like material with progressive thnning; the rhTGF-β1 incubated group had accelerated chondrogenesis but also accelerated degeneration. The rhTGF-β1 injected groups did not enhance chondrogenesis but generated osteophytes The non-grafted group only healed with non-cartilaginous material 　　The rhTGF-β1 pre-incubation in vitro is preferred over intra-articular injection as a delivery vehicle of this polypeptide growth factor and is effective in acce erating cnoncrogenesis, but early degeneration of new cartilage necessitate further study.