Proteins Recovery by Miscellaneous Precipitation Methods

• Main Authors: K''un - Hsing Hsiao, 蕭坤星
• Other Authors: Professor T''e - Liang Ch''en
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/07788444622259269558

碩士 === 國立成功大學 === 化學工程研究所 === 83 === In biotechnology, the recovery of proteins from a fermentation broth by precipitation is a primary focus of many downstream processing unit operations, since it serves as both the concentration and purification steps. The efficiencies of protein removal and fractionation are affected by protein solubility directly. Many factors affect the apparent equilibrium solubility and most of them have been studied already. However, the influence of the initial protein concentration is often neglected. In this work, we used ammonium sulfate, acetone, ethanol, polyethylene glycol (PEG) and carboxymethylcellulose (CMC) to precipitate lysozyme, ?- chymotrypsin, albumin of bovine and amyloglucosidase in a batch magnetic stirred reactor. The results showed that the influence of the initial protein concentration was dependent on type of protein and method of precipitation. The mechanisms involved were also different from each other. In addition, the recovery of amyloglucosidase by various precipitants was examined. The efficiency of recovery was found to be PEG > ammonium sulfate > > ethanol > acetone, but the effect of concentration was acetone > ethanol > PEG > ammonium sulfate.