| Summary: | 碩士 === 國立成功大學 === 化學工程研究所 === 83 === In biotechnology, the recovery of proteins from a fermentation
broth by precipitation is a primary focus of many downstream
processing unit operations, since it serves as both the
concentration and purification steps. The efficiencies of
protein removal and fractionation are affected by protein
solubility directly. Many factors affect the apparent
equilibrium solubility and most of them have been studied
already. However, the influence of the initial protein
concentration is often neglected. In this work, we used
ammonium sulfate, acetone, ethanol, polyethylene glycol (PEG)
and carboxymethylcellulose (CMC) to precipitate lysozyme, ?-
chymotrypsin, albumin of bovine and amyloglucosidase in a batch
magnetic stirred reactor. The results showed that the
influence of the initial protein concentration was dependent on
type of protein and method of precipitation. The mechanisms
involved were also different from each other. In addition, the
recovery of amyloglucosidase by various precipitants was
examined. The efficiency of recovery was found to be PEG >
ammonium sulfate > > ethanol > acetone, but the effect of
concentration was acetone > ethanol > PEG > ammonium sulfate.
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