Effect of choral polymer on Lipase catalyzed transesterification

• Main Authors: Scott Wu, 吳阜蒼
• Other Authors: Professer Jui-Hsiang Liu
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/72313640933547281625

碩士 === 國立成功大學 === 化學工程研究所 === 83 === Resolution of racemic (±) 1-phenethyl alcohol via PPL(porcine pancreatic lipase) in organic solvents by transesterification with vinyl acetatet was carried out in this investigtion。 Solvent effect on the stereospecificity of the enzyme was investigated。It was found that the reaction rate have a good correlation with hydrophobicity constant log P，and the reaction rate increased with increasing the polarity of solvents 。 PPL at higher temperature stil have good thermal catalysis， this shows that the PPL has a better stability in organ ic solvent than in water。 PPL in water saturated n-heptane have higher reaction rate and lower enantioselectivity than in dehydrated n-heptane。In triethyl amine,howeve,added of water caused the decreasing of PPL activity and the enantioselectivity almost not be affected。 In the presence of chiral polymer TDI-CPO,the reaction system in tetrahydrofurane was found that the reaction rate and the enantiomeric ratio of the system were both increrased。