The research of production and release of creatinase by recombinant E.coli

• Main Authors: Chang Ming-chen, 張銘琛
• Other Authors: Cheng Chu-Yuan
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/93645628224330073792

碩士 === 國立成功大學 === 化學工程研究所 === 83 === The feasibility of extracellular production of creatinase by recombinant Escherichia coli was investigated. First, the effect of addition of IPTG was examined. The result was shown that the optimal condition for induction was obtained when 0.5mM IPTG was added at fermentation time 1 hours.Secondly it wasindicated that the release of creatinase with culture broth was more obvious in C/N=0.1 than the other ratios. Nextly, it was demonstrated the extent of creatinase release increased with EDTA concentration.Furthermore,the maximum extracellular creatinase activity was achieved when 3mM EDTA was added after 3hours.at the same condition,the extent of creatinase realease reached to 33.7%.In the continuous production of creatinase , it was indicated creatinase decreased from 10.3 to 7.6U/mL as the dilution rate increased from 0.05 to 0.1.The continuous operation with EDTA,17.8% creatinase was released into medium with total activity 8.5U/mL at dilution rate 0.05.