| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 83 === The objective of this study is to identify the genotypes of
milk protein genetic markers in dairy bulls. The genotype and
predicted transmitting ability of dairy bulls were compared,
and using random amplified polymorphic DNA polymerase chain
reaction (RAPD PCR) to establish the DNA fingerprints of dairy
bulls. Kappa-casein genotypes of 43 dairy bulls were analyzed
using PCR with restriction fragment length polymorphisms (PCR-
RPLPs), and the genotypes identified were 35 AA, 7 BB, and 1
BB. The B gene frequency was found to be 10.5%. Comparison
between genotypes and transmitting trait evaluation indicated
that bulls with AB type gene have higher predited transmitting
ability for the yield of milk, protein and fat than those with
BB type gene. In the alpha-lactalbumin genotyping of 45 dairy
bulls, the genotypes identified were 2 AA, 18 AB, and 25 BB.
The A gene frequency was found to be 24.4%. Comparison between
genotypes and transmitting trait evaluation indicated that
bulls with AA type and AB type gene have higher predicted
transmitting ability. DNA fingerprints were established by RAPD
PCR. By screening with 140 primers, 16 fingerprints and 43
stable amplified products were obtained. The stability of the
fingerprints was confirmed by repeating the tests for
individual and pooled semen DNA. The results of this study can
serve as a reference for dairymen to select the frozen semens
of dairy bulls. The establishment of DNA fingerprints may also
contribute to the improveement in the selection of dairy cattle.
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