Changes of Amino Acids and Polyamines in Desiccation-tolerant Mechanism in Chlorella

• Main Authors: Ling-Ling Chang, 張凌凌
• Other Authors: Pei-Chung Chen
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/76178708681216486571

碩士 === 國立中興大學 === 植物學系 === 83 === By using the regular light-dark transition to make Chlorella sp. strain DT (DT) and strain 211-8b (8b) acheive the synchronous cell cycle. After desiccation,the total amount of amino acids of both strains decreased, but the amount of DT was always higher than that of 8b. The total amount of free poly- amines in the clells of DT and 8b increased in coordination with the cells during the cell cycle, until the 20th hour reached the heighest amount. The cells of DT in every stage were dried 2 hours at 30℃, then dried 1 hour at 42℃, it was found that the total amount of polyamines in DT cells increased, especially the cell of the 20th hour, the total amount of polyamines increased 10.7 times more than which in untreatment cells. And the cells of 8b in every stage were applied the same treatment, the total amount of polyamines in cells was decreased. However, at the 20th hour, the amount of polyamines increased two times comparison of other stages. But the increasement of 8b was far less than which of DT. After dried DT cells with higher temperature at 60℃, the total amount of polyamines decreased. Therefore we assumed that the accumulation of polyamines after drying treatment is possibly related to the osmoregulation of cell in desiccation.