The preservation of stock culture off Skeletonema costatum

• Main Authors: L.W. Lee, 李立文
• Other Authors: I.C. Hsiao
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/11273101883008815141

碩士 === 東海大學 === 生物學研究所 === 82 === Skeletonema costatum(Greville)Cleve grows rapidly to reache al cultures.Soon after reaching its peak,it declines withoutonary phase.To maintain its exponential phase, it is necessary touently for the purpose of the preservation of stock culture. Experimentst to reduce their metabolic activites and induce the formation ofy various combinations of light,temperature nutrients,anaerobic conditions.At 15℃in the dark for 18 days, the Skeletonema cellscytoplasmic contents and their shapes appeared similar to thosecells.After 33 days,they were still viable and could rejuveate in ditions.These cellscould extendtheir exponential phase for morewhen they were illuminated with 32uEm-2S-1 of intensity on aight and 22.5h dark.Different wavelengths of light did not inducef resting cells in S.costatum,but influencedtheir growth rates.Therogen and phosphrous did not induce the formation of restingeir vegetative cells shrivelled up when grown in a nitrogenm.They turnedpaleand lost their chloropasts in the phosporousdeficientdays after being kept in these conditions, most of them wereed by bacteria of dead.In an oxygen-depleted medium,thelsshowed no di- fference from those which were grown in the oxygen-sufficientThey lost their rejuvenescence even after the medium wash oxygen.In the desiccation experiment using differenteSkeleto-nema cells couldnot rejuvenated after being returned to their standardons.