A Study on the Determination of Copper(II) and Cobalt(II) by Ion-pair Chromatography

• Main Authors: Wen-Fang Chang, 張文芳
• Other Authors: Suh-Jen Jane Tsai
• Format: Others
• Language: zh-TW
• Published: 1994
• Online Access: http://ndltd.ncl.edu.tw/handle/41411036737196992771

碩士 === 靜宜大學 === 應用化學系 === 82 === Since metals and their complexes play an important role in both biological chemistry and in enviromental chemistry, the analysis of metals is rather important. In recent years, high performance liquid chromatography coupled with various detectors has become one of the best methods for metal analysis. In this study, we used 4-（2-pyridylazo）resorcinol (PAR） as a pre-column reagent, Cu-PAR and Co-PAR were separated and quantitatively determined by reverse phase high performance liquid chromatography with UV-VIS detector. We also investigated the absorption and chromatographic characteristics of these metal chelates. The optimum condition established in this work was:acetonitrile/methanol /tartrate buffer (pH=6.5)= 6/30/64 including 1.0×10▋▋ ion-pair reagent( tetrabutylammonium bromide) The separation was done with C▋▋ eversed phase column. The proposed method was appilied to determine Cu and Co in a relatively complex substance, namely, nickel-base superalloy. The precision and accuracy of the proposed chromatographic method were evaluated。